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Fluorescent DNA Fragment Analysis
General Information
- Instrumentation: Applied Biosystems 3730 Genetic Analyzer
- Supported Fluorescent Dye Labels (G5 matrix): 6FAM VIC NED PET
- Size Standard: LIZ 500; ability to size fragments 50-500 bp within +/- 0.5
- Service Includes: Preparation of your PCR samples using HiDi Formamide with LIZ 500 size standard and capillary electrophoresis run on the ABI 3730
Optional Sample Preparation Services *
*Note: The optional sample preparation services are not included in the Genetic Fragment Analysis Pricing. See our Pricing Page for complete information.
Sample Submission
- Fluorescent labeled PCR samples must be pooled and/or diluted prior to submission; submit 2uL to FGC in an Abgene 96-well PCR plate or in 1.5mL microfuge tubes (Please contact Michelle Zanche if you are a first time user. The FGC can assist you in optimizing sample dilutions.)
- Complete Fragment Analysis Request Form, place samples and form in a Ziploc bag and drop-off in 4C Refrigerator (Medical Center Ground Floor Room G-7814)
Data Retrieval and Analysis
- FGC staff will email your .fsa data files for your analysis
- ABI GeneMapper software is necessary to analyze the .fsa files; the software is available at no charge on the FGC public workstation. For instructions on how to access FGC's public workstation go to our General Information page.
- For assistance using GeneMapper software please contact Michelle Zanche
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