Genotyping - PCR Amplification and Gel Electrophoresis

• Samples for genotyping via PCR amplification and gel electrophoresis may be submitted as tissue* or as purified DNA
• Customers are responsible for designing and optimizing PCR assays and providing the primers to the FGC (see optional services below if you require assistance)
• Service Includes: PCR amplification, agarose gel electrophoresis and genotyping calls (if applicable)

---

Optional Sample Preparation Services *

• DNA Extractions
• Please inquire if you need assay design/optimization services

*Note: The optional sample preparation services are not included in the PCR Amplification and Gel Electrophoresis Pricing. See our Pricing Page for complete information.

---

Sample Submission

• If submitting tissue: Place samples vertically (A1, B1, C1..) in an Abgene 2.2mL 96 deep-well plate and seal tightly with adhesive foil seal.
• If submitting purified DNA: Place samples either vertically in an Abgene 96 well plate sealed tightly with adhesive foil seal or in 1.5mL microfuge tubes
• Complete Genotyping Sample Registration Form; fill in appropriate information indicating whether you are submitting tissue or DNA, and what assays you need run
• Place samples and form in a Ziploc bag and drop-off in 4C Refrigerator (Medical Center Ground Floor Room G-7814)

---

Data Retrieval and Analysis

• FGC staff will email your gel image and genotyping results in an Excel spreadsheet