Real Time PCR - FGC Setup and Instrument Run

• Instrumentation: Applied Biosystems 7900HT Sequence Detection System; Corbett Robotics CAS-1200 precision liquid handling system for plate set-up.
• Applications: Absolute Quantification, Relative Gene Expression, and Allelic Discrimination for SNP genotyping
• Available Formats: 384-well plate or ABI Taqman Low Density Array (microfluidic card); see list of pre-made TLDA cards
• Customer Provides: Probes and primers
• Service Includes: Setup of real time PCR reactions using ABI Taqman or SYBR Green master mix and instrument run

Optional Assay Order Services*

• Primers and probes can be special ordered through Applied Biosystems (ABI) and Integrated DNA Technologies (IDT)
• In-stock probes: Roche Universal Human, Mouse and Rat Probe Library (Formerly Exiqon )
• Order requests can be placed through David Terry

Optional Sample Preparation Services*

• RNA extractions (includes Bioanalyzer analysis)
• Bioanalyzer analysis of customer prepared RNA
• cDNA synthesis (requires previous Bioanalyzer analysis)
• RNA amplification (NuGEN WT-Ovation)
• Real Time PCR assay optimization and validation

Sample Submission

• Please submit appropriate form with your samples

Data Retrieval and Analysis

• Click for INSTRUCTIONS on using Real Time PCR LIMS to download your data
• Data Analysis